TBE buffer
TBE buffer is a solution of Tris base, boric acid and EDTA.
This buffer mostly used in DNA electrophoresis experiment.
Recipe for 10x TBE (TrisBorateEDTA) buffer:
--------------------------------------------
Tris base :107g
Boric acid: 55g
0.5M EDTA : 40ml
Add distilled water and adjust the volume to 1 liter.
TE buffer
TE buffer protects DNA or RNA from degradation.
Recipe 1xTE buffer:
--------------------
1ml of 1M Tris-HCL (pH 7.5-8)
0.2ml of 0.5M EDTA (pH 8)
add 988ml of distilled water to make 1 liter 1x TE buffer.
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