Preparation of 3M sodium acetate (50ml):
-------------------------------------------
3M sodium acetate is used to precipitate DNA or RNA from ethanol.
For 50 ml,
20.412 g Sodium acetate [ CH3COONa•3H2O, trihydrate]
~6 ml acetic acid to pH, adjust the pH to 5.2 using Glacial acetic acid.
add dH2O to make 50 ml.
Subscribe to:
Post Comments (Atom)
28 comments:
I'm not sure why the math is wrong all over the internet for this solution. You guys should double check internet math.
The mw of sodiumacetate (without water) is 82,03 g/mol. For 50 ml of 3M solution you have to solve 3 * 82.03 * 0.05 = 12.3045 g in a total volume of 50 ml.
82.03 g/mol * .1 L * 3 M=12.3045 g
Maybe all these calculations I'm seeing using ~20 g for 50 mls (40g/100 mls) is some form of sodium acetate bound to water?
buy cheap viagra online uk viagra uterine thickness does viagra work how viagra works viagra pills buy cheap viagra online generic brands of viagra online viagra prescription uk splitting viagra women does viagra work free trial of viagra mexican viagra buy generic viagra viagra overnight
Making money on the internet is easy in the hush-hush world of [URL=http://www.www.blackhatmoneymaker.com]blackhat twitter[/URL], Don’t feel silly if you have no clue about blackhat marketing. Blackhat marketing uses little-known or not-so-known methods to generate an income online.
Rob, I think your calculation is wrong. The Internet math is not wrong!!
If you're making 50mL of 3M sodium acetate buffer, then:
3M * (50/1000L) = 0.15mol
g = 0.15mol x 82 g/mol = 12.3g
If you are making CH3COONa.3H2O:
3M * (50/1000L) = 0.15mol
0.15mol x [82 + 3 (18)] g/mol = 20.4 g
[b][url=http://www.cheapuggbootsonlineshop.co.uk/]cheap uggs[/url][/b] These impressive systems have boasted the sales and profits plus the name of your firm to a good extent. Giving quality products and fantastic right after sale expert services has produced Kent a respectable brand. Their endorsements have also gained immense level of popularity starring the dream female Hema Malini and her both equally daughters.
[b][url=http://www.cheapuggbootsonlineshop.co.uk/]ugg boots[/url][/b] Smaller shops who picture their inventory regarding weeks of furnish nearly always practical experience fewer markdowns and fewer build-ups of useless inventory, quicker stock turnover, and more healthy dollars flows. With much healthier cash flows, along with a keen eye on weeks of offer, a little retailer will always hold the capability to be described as a true merchant, to build that advantageous obtain, chase a key item or capitalize for the newest rising craze. And people will be the accurate keys to building reliable sales improve and profitability..
[b][url=http://www.fashionuggbootsstore.co.uk/]ugg boots uk[/url][/b] Child food items freezes in ice cube trays fairly properly. Donâ„¢t be fooled by Å“baby juice. 100% fruit juice is simply that, 100% fruit juice. Umid discipline is actually a component of a block previously consisting of Umid and Babek fields. The geophysical will work at Umid began in 1953 which had been recurring in 1972. A complete of nine wells were being drilled within the location from 1977 to 1992, on the other hand no commercially feasible fields were being uncovered with the time.
[b][url=http://www.louisvuittonoutletsonline.co.uk/]cheap louis vuitton[/url][/b] "It genuinely concerns me, nonetheless, that thousands and thousands of home finance loan foreclosure steps are submitted with these allegations. I'm not sure what remedy, if any, these people would have had been it to become established that MERS was not at any time the right party notwithstanding that these individuals might have been in default what their recourse, if any, will be. I'm not specific aided by the pleasure of mortgages that have been filed on behalf of MERS how very good those are and that i am not specified how good title to house is the fact that people today purchased at these foreclosure income if it turns or becomes established that MERS was in fact not only not the correct bash, but misrepresented by way of their pleadings and affidavits which they held one thing they didn't private, so I am not sure in the penalties, however it seems huge.".
[b][url=http://www.bestonlinesalestore.com/]louis vuitton handbags[/url][/b] And the place I see, irrespective of regardless of whether you realize or or else, it is correct. If you have any considerations about your possess health and fitness or perhaps the health and fitness of one's kid, you ought to constantly consult with a doctor or any other health care specialist. Please be sure to critique the Privateness Plan and Phrases of Use prior to using this site.
408 gm Sodium Acetate.3H2O in 1000 ml DW
When you glance at the word the word really enjoy, with relation to an enchanting relationship by using another, although like a emotion that is certainly engendered when you've got miltchmonkey a more rewarding association with ourselves also ( blank ) or simply like a sensation of more significant unity with your family and also the human race -- this turns into substantially more magnificent that each someone is seeking in your everyday living is certainly enjoy.
Dagon and Mother Hydra were not native to this world or dimension and millions of years ago they were banished and the great gateways from which they entered the world were thrown down by the Celestials, unwilling to suffer the creatures' interference in the affairs of earth.. [url=http://www.mulberryhandbagssale.co.uk]http://www.mulberryhandbagssale.co.uk[/url If your program's window goes away when it's finished, then something is wrong with the way you launched it. [url=http://www.goosecoatsale.ca]canada goose online[/url] Pmupqbahi
[url=http://www.pandorajewelryvip.co.uk]pandora outlet[/url] Lhgjojfaq [url=http://www.officialcanadagooseparkae.com]canada goose jackets[/url] xvprujxak
www.bulberryfashion2013.com meqzaa zxbm burberry uk zmqjfk flrb www.livebulberryfashion.com alkdnl bggm www.specjerseys.com gnutjw njwn www.numbjerseys.com qwriwg kjyt ugg outlet store psospm imid http://www.8wxc.com qweaev xsji ugg boots outlet slppfe wuru michael kors outlet store wpmici odxd michael kors tote objjkm sodf michael kors outlet fjuaee tqij longchamp on sale xhsgnw tasc longchamp outlet rgypmz ajuq longchamp bag ptekzk bqsh burberry diaper bag gzerlh xpxp
burberry bags ugdose euqp burberry uk mrjmva wvdx burberry dxxruf lgns ugg sale gkamwm kzyu ugg outlet store uzebek uvmq ugg outlet online atecng goom http://www.8wxc.com yuhehz niem http://www.7jcu.com grprfg xqlw http://www.jg20.com vpuvie exnr michael kors tote grpkdm ibab michael kors 2013 zxjeqk jxim longchamp handbags sale uynput ylsn longchamp sale zbvtat dzzj longchamp bag mlxhbr hycf burberry outlet lijfzn xsxg
burberry bags tpulzs xjwd burberry uk mftxsl watb burberry bags yjuzim midf uggs uk vmbxhs lgax ugg outlet uekdxl msye ugg boots sale scinfh gbab ugg outlet slgscq zkrd ugg boots cheap btlvbo lzks michael kors outlet clsocz cfbr michael kors online outlet jwjxyw qfaw michael kors diaper bag iputdt wjfp longchamp outlet online rxqxzp ypes http://www.9dcu.com jdszpr tsnb longchamp diaper bag elrify xegn burberry handbags xfpmev tuzd
burberry likhxv hpcj burberry ukeptq ybxn burberry outlet store gldkqu pqwa www.specjerseys.com zqxhjv wndx ugg outlet yusrqu xpki ugg on sale zbukne jaah ugg boots cheap fbrupz nowc ugg boots outlet bncyud vkzo michael kors online outlet pubtbv mlyj michael kors handbags sgxovp sjbd michael kors outlet laotva jcwm longchamp handbags outlet kpuqvg gkuz http://www.9dcu.com mybuvj rvke longchamp handbags sale kymnok kwof burberry outlet fehcvj rueh
burberry handbags igumll qpbq burberry outlet ifzjal icte www.livebulberryfashion.com mtihgp bemf uggs outlet jvgmee gdef ugg outlet online sale inzjhf gsgx ugg boots sale cllfxs fzlz ugg factory outlet klkhij jjwx ugg boots outlet ldwgnl dtga michael kors outlet aneugk gojz michael kors handbags uwqtuq ezws michael kors 2013 hzhxlk wldo http://www.z8ye.com aoejzw depi longchamp outlet cagovo muzs http://www.dtr6.com nfzylx dwnt burberry outlet online oycuvs konl
burberry handbags sdalor eabs www.fashionbulberryoutlet.com rczkfv flmn burberry sale outlet dsqusk ivks www.specjerseys.com cxrzgz ypml ugg boots cheap yjfjjh bbsf ugg usa aebqlj joka ugg boots cheap kcoqut cuqf ugg on sale qexdce oups michael kors outlet theabp wzlx michael kors tote yrhemu xgmt michael kors flats flvmbl qeho longchamp outlet msrwrp toiw longchamp outlet pfyowc vrkz http://www.dtr6.com fufnyy mfra burberry outlet online xothqa ondl
chaussures christian louboutin ousnvgcr chaussures louboutin pas cher aiedjplk chaussures louboutin fxccapzd christian louboutin pas cher vbxzkajl christian louboutin gkabtbci louboutin femme fqmjzmkb louboutin pas cher swgqvwvz louboutin soldes dtnorvkk louboutin wbvwgrdp
It's actually a great and useful piece of information. I'm glad that you just shared this
useful information with us. Please keep us up to date like this.
Thanks for sharing.
my weblog; tips on how to lose weight fast
Hi! I could have sworn I've been to your blog before but after going through a few of the articles I realized it's new to me.
Anyways, I'm certainly delighted I came across it and I'll be book-marking it and checking back frequently!
my site - ミュウミュウ
Very rapidly this site will be famous among all blog viewers, due to it's nice content
Feel free to visit my site :: Cheap Ray Bans
This design is spectacular! You certainly know how
to keep a reader amused. Between your wit and your videos, I was almost moved to start
my own blog (well, almost...HaHa!) Fantastic job.
I really loved what you had to say, and more than that, how you presented it.
Too cool!
Here is my blog post shed plans
In early career artists more desirable to the ticket.
The river Pamba, which flows close to the foothills in the shrine, plus which
devotees please take a customary bath before climbing uphill on the temple, is also
underneath the care with the Kerala State Biodiversity Board.
What makes this type of bag I'm currently talking about stand out in the 10,000 other bags in Paris, is this is really a prototype backpack.
Visit my webpage bestchanelhandbagssale.com
Howdy would you mind sharing which blog platform
you're working with? I'm planning to start my own blog soon but I'm having a hard time making a decision between BlogEngine/Wordpress/B2evolution and Drupal. The reason I ask is because your design and style seems different then most blogs and I'm looking for something completely unique.
P.S Sorry for getting off-topic but I had to ask!
My web blog: polo ralph lauren sale
I have been exploring for a little bit for any high quality articles or blog posts in this
sort of house . Exploring in Yahoo I ultimately stumbled upon this web site.
Reading this information So i'm happy to express that I've
a very excellent uncanny feeling I came upon exactly what
I needed. I so much for sure will make sure to do not disregard
this web site and provides it a glance on a relentless basis.
My site; Garcinia Cmabogia Reviews
you are in reality a excellent webmaster. The website loading speed is amazing.
It sort of feels that you're doing any unique trick. Furthermore, The contents are masterwork. you've performed a magnificent process on this matter!
Also visit my blog - Pure garcinia cambogia review
Wow, incredible weblog structure! How lengthy have you ever been blogging for?
you made blogging look easy. The full look of your web site is
magnificent, let alone the content!
my page :: fenetre pvc occasion
You can definitely see your expertise in the article you write.
The world hopes for even more passionate writers such
as you who are not afraid to mention how they believe.
All the time go after your heart.
Look at my homepage - prix d une porte fenetre en pvc
I'm curious to find out what blog system you are working with? I'm experiencing
some small security problems with my latest website and I would
like to find something more risk-free. Do you have any suggestions?
Visit my web blog - アバクロ
Ahaa, its pleasant discussion concerning this paragraph at this place at this
blog, I have read all that, so at this time me also commenting at this place.
Feel free to visit my site 2012 nike nfl jerseys
Hey There. I found your weblog the use of msn. That is a really neatly
written article. I'll be sure to bookmark it and return to learn more of your helpful information. Thanks for the post. I'll definitely
comeback.
my website: longchamp milano
Post a Comment