Showing posts with label TE. Show all posts
Showing posts with label TE. Show all posts

Tuesday, September 9, 2008

TBE and TE buffer preparation

TBE buffer
TBE buffer is a solution of Tris base, boric acid and EDTA.
This buffer mostly used in DNA electrophoresis experiment.

Recipe for 10x TBE (TrisBorateEDTA) buffer:
--------------------------------------------
Tris base :107g
Boric acid: 55g
0.5M EDTA : 40ml
Add distilled water and adjust the volume to 1 liter.

TE buffer
TE buffer protects DNA or RNA from degradation.

Recipe 1xTE buffer:
--------------------
1ml of 1M Tris-HCL (pH 7.5-8)
0.2ml of 0.5M EDTA (pH 8)
add 988ml of distilled water to make 1 liter 1x TE buffer.